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  19 April 2014  
Yeast Metabolic Engineering: Methods and Protocols (Methods in Molecular Biology, Book 1152)

Yeast Metabolic Engineering: Methods and Protocols (Methods in Molecular Biology, Book 1152) by Valeria Mapelli
English | 2014 | ISBN: 1493905627 | ISBN-13: 9781493905621 | 316 pages | PDF | 5 MB

Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. 

Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.

  19 April 2014  
Artisanal Gluten-Free Cupcakes: From-Scratch Recipes to Delight Every Cupcake Devotee?Gluten-Free and Otherwise
Artisanal Gluten-Free Cupcakes: From-Scratch Recipes to Delight Every Cupcake Devotee?Gluten-Free and Otherwise 
English | 2011 | ISBN: 1615190368 | 272 pages | PDF | 101 MB
  18 April 2014  
ng-book - The Complete Book on AngularJS Training Video

ng-book - The Complete Book on AngularJS Training Video
English | .MP4 | Audio: aac, 44100 Hz, stereo | Video: h264, yuv420p, 1308x754, 30.00 fps(r) | 705 MB
Genre: E-learning

The in-depth, complete, and up-to-date book on Angular. Become an AngularJS expert today.
  18 April 2014  
The New Baby Answer Book: From Birth to Kindergarten, Answers to the Top 150 Questions about Raising a Young Child

The New Baby Answer Book: From Birth to Kindergarten, Answers to the Top 150 Questions about Raising a Young Child by Robin Goldstein Ph.D., Janet Gallant
English | 2009 | ISBN: 1402218273 | 288 pages | PDF | 4,5 MB


Is my baby "good"? Should I pick my baby up when he cries? What's the best way to introduce a new baby to an older sibling? Is co-sleeping with my child okay? Am I spoiling my child? How can I convince my child to try new foods? What should I do when my child argues with her friends? How do I encourage learning at home?

  18 April 2014  

The Hobbit The Desolation of Smaug 2013 1080p Bluray Dual Audio [HINDI 6CH+ENGLISH 6CH] AC3 x264-Gan...



The Hobbit The Desolation of Smaug 2013



Language: English | Hindi
Size: 7376399121 bytes (6,87 GiB), duration: 02:41:17, avg.bitrate: 6098 kb/s
Audio: ac3, 48000 Hz, 5:1
Audio: ac3, 48000 Hz, 5:1
Video: h264, yuv420p, 1920x800, 23,98 fps(r)
Subtitles: eng chi chi
Genre: Adventure | Drama | Fantasy
IMDB info



The dwarves, along with Bilbo Baggins and Gandalf the Grey, continue their quest to reclaim Erebor, their homeland, from Smaug. Bilbo Baggins is in possession of a mysterious and magical ring.

  18 April 2014  

The Book Thief 2013 BRRIP x264 AC3-BTRG



The Book Thief 2013



Language : English / German
Size: 1970951990 bytes (1,84 GiB), duration: 02:10:59, avg.bitrate: 2006 kb/s
Audio: ac3, 48000 Hz, 5:1 (eng)
Video: h264, yuv420p, 720x304, 23,98 fps(r)
Genre: Drama / War
IMDB info



While subjected to the horrors of World War II Germany, young Liesel finds solace by stealing books and sharing them with others.

  18 April 2014  

RED 2 (2013) 720p BrRip x264 [Dual Audio] [Hindi-English]-Ganool



RED 2 (2013)



Language : English / Hindi
Size: 1048600846 bytes (1000,02 MiB), duration: 01:55:48, avg.bitrate: 1207 kb/s
Audio: aac, 48000 Hz, stereo (hin)
Audio: aac, 48000 Hz, 5:1 (eng)
Video: h264, yuv420p, 1280x536, 23,98 fps(r)
Genre: Action | Comedy | Crime | Thriller
IMDB info



Retired C.I.A. agent Frank Moses reunites his unlikely team of elite operatives for a global quest to track down a missing portable nuclear device.

  18 April 2014  
300 Rise of an Empire 2014 720p HD Hindi English Dual Audio


300 Rise of an Empire 2014 720p HD Hindi English Dual Audio


Language : English | Hindi
Size: 1037760635 bytes (989,69 MiB), duration: 01:40:09, avg.bitrate: 1382 kb/s
Audio: mp3, 48000 Hz, stereo (hin)
Audio: aac, 48000 Hz, stereo (eng)
Video: h264, yuv420p, 1280x526, 23,98 fps(r) (eng)
Genre: Action | Drama | War
IMDB info


Greek general Themistokles leads the charge against invading Persian forces led by mortal-turned-god Xerxes and Artemisia, vengeful commander of the Persian navy.

  18 April 2014  
The Hobbit The Desolation of Smaug [2013] 1080p BRRip [Dual Audio] [English + Hindi] AAC x264 BUZZcc...


The Hobbit The Desolation of Smaug [2013] 1080p BRRip [Dual Audio] [English + Hindi] AAC x264 BUZZccd


Language: English | Hindi
Size: 2368641977 bytes (2,21 GiB), duration: 02:41:17, avg.bitrate: 1958 kb/s
Audio: aac, 48000 Hz, stereo (eng)
Audio: aac, 48000 Hz, stereo (hin)
Video: h264, yuv420p, 1920x800, 23,98 fps(r) (eng)
Genre: Adventure | Drama | Fantasy
IMDB info


The dwarves, along with Bilbo Baggins and Gandalf the Grey, continue their quest to reclaim Erebor, their homeland, from Smaug. Bilbo Baggins is in possession of a mysterious and magical ring.

  18 April 2014  
Tinker Bell and the Great Fairy Rescue [2010] 720p BRRip [Dual Audio] [English + Hindi] AAC x264 BUZ...


Tinker Bell and the Great Fairy Rescue [2010] 720p BRRip [Dual Audio] [English + Hindi] AAC x264 BUZZccd


Language : English | Hindi
Size: 799317815 bytes (762,29 MiB), duration: 01:16:27, avg.bitrate: 1394 kb/s
Audio: aac, 48000 Hz, stereo (hin)
Audio: aac, 48000 Hz, stereo (eng)
Video: h264, yuv420p, 1280x720, 23,98 fps(r) (hin)
Subtitles: eng
Genre: Animation / Fantasy
IMDB info


When a misunderstood dust-keeper fairy named Zarina steals Pixie Hollow's all-important Blue Pixie Dust, and flies away to join forces with the pirates of Skull Rock, Tinker Bell and her fairy friends must embark on the adventure of a lifetime to return it to its rightful place. However, in the midst of their pursuit of Zarina, Tink's world is turned upside down. She and her friends find that their respective talents have been switched and they have to race against time to retrieve the Blue Pixie Dust and return home to save Pixie Hollow.

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