Become the iconic hero, Astro Boy, and take to the streets and skies on an epic adventure to save Metro City from the clutches of the evil President Stone and his robot army. Utilizing Astro's entire arsenal of iconic weapons and abilities -- Finger Lasers, Arm Cannon, Butt Machine Guns and Drill Attacks, players will battle hordes of robot enemies and colossal bosses including some never-before-seen enemies across Metro City and the mysterious Surface. Using an intuitive combat system that adds depth to gameplay, players will experience classic platform action and exhilarating flight combat levels as they use Astro's unique rocket boosters to hover, avoid enemies and zip out of the way of danger. Astro Boy offers numerous unlockable and collectible elements that include exclusive art work, different versions of Astro Boy and more, as players live the story solo or side by side with a friend in 2 player co-op mode on Wii.

The game is essentially an isometric beat-em-up, although Jackson attacks with magic powers instead of physical contact, and has the ability to shoot magic power at enemies instead of getting close enough for a melee attack. A map of the stage is shown before it begins, and after which, Jackson must get from the start to the end without losing all his health, rescuing all the children and defeating all the enemies along the way.
The game can also be played multiplayer; if the cabinet supports it, up to three people can play simultaneously. All three players play as Jackson, dressed in his suit from the "Smooth Criminal" music video. The first player wears a white suit and hat, with a blue shirt; the second player's character dons a scarlet outfit with a black shirt; the third player's character is dressed in black, with a red shirt. The characters all have armbands: red for black outfit, black for red outfit, and blue for white outfit.